Faculty Interests Database

Faculty Affairs: Faculty Interests Database Theodore F. Taraschi, PhD

Pathology, Anatomy & Cell Biology
Professor and Vice Chair of Education

Associate Dean for Research, Jefferson Medical College

Mailing Address Contact Information
1020 Locust Street JAH 209
Philadelphia, Pennsylvania 19107
United States
Phone: (215) 503-5020
Ph.D., Rutgers University, Chemistry, 1980

Expertise and Research Interests
The invasion of erythrocytes by Plasmodium falciparum parasites produces aninfection that causes major changes in the structure, composition, antigenicity and function of the host cell membrane. During infection, the parasite actively imports obligate nutrients from the serum and exports lipids, proteins and membranes to the erythrocyte cytoplasm and the erythrocyte membrane. The regulation of these pathways must be underparasite control since mature erythrocytes have limited lipid synthetic or metabolic capabilities and do not have the machinery to synthesize or process proteins. Elucidation of trafficking pathways is complex in Plasmodia-infected erythrocytes compared to other eukaryotic cells, since the parasite is separated from the serum by its plasma membrane, the parasitophorous vacuolar membrane PVM and the erythrocyte membrane. There remains a long-felt need to identify the cellular components of these trafficking pathways to gain a better understanding of transport mechanisms in malaria-infected erythrocytes.

The laboratory is engaged in three areas of inquiry:

1) Elucidation of parasite protein trafficking pathways from intracellular parasites to the erythrocyte cytosol and host cell membrane.

2) Determinantion of the mechanism of hemoglobin uptake and transport by intraerythrocytic parasites

3) Characterization of parasite DNA repair pathways (e.g. base excision and mismatch repair).
Actin; Confocal Scanning Microscopy; Cytoskeleton; DNA repair; Electron Microscopy; Erythrocytes; Endocytosis; Fluorescence Microscopy; hemoglobin uptake; Immunoelectron Microscopy; Malaria; Microtubules; Plasmodium falciparum; Protein trafficking; Transfection; Vesicle transport
  • Pubmed
  • Frankland, S., Adisa, A., Horrocks, P., Taraschi, T.F., Schneider, T., Elliott, S.R., Rogerson, S.J., Knuepfer, E., Cowman, A.F., Newbold, C.I. and Tilley, L. (2006) Delivery of the Malaria Virulence Protein, PfEMP1, to the Erythrocyte Surface Requires Cholesterol-Rich Domains. Euk. Cell (In Press).
  • Komatsu, H., Westerman, J., Snoek, G.T., Taraschi, T.F. and Janes, N. (2004) Effects of D-myo-inositol 1-phosphate on the transfer function of phosphatidylinositol transfer protein a. J. Liposome Research 14, 141-153.
  • Komatsu, H., Westerman, J., Snoek, G.T., Taraschi, T.F. and Janes, N. (2003) L-alpha-glycerylphosphorylcholine inhibits the transfer function of phosphatidylinositol transfer protein alpha. Biochim. Biophys. Acta 1635, 67-74.
  • Taraschi, T.F., ODonnell, M., Martinez, S.L., Trelka, D., Tilley, L., Fowler, V., Moriyama, Y., and Schneider, T.G. (2003) Generation of an erythrocyte vesicle transport system by Plasmodium falciparum malaria parasites. Blood 102, 3420-3427.
  • Taraschi, T.F., Trelka, D., Martinez, S., Schneider, T. and ODonnell, M.E. (2001) Vesicle-mediated trafficking of parasite proteins to the host cell cytosol and erythrocyte surface membrane in Plasmodium falciparum infected erythrocytes. Int. J. Parasitol. 31,1381-1391.
  • Nicolas, E., Beggs, J.M. and Taraschi, T.F. (2000) Gelonin is an unusual DNA glycosylase that removes adenine from single-stranded DNA, normal base pairs and mismatches. J. Biol. Chem. 275, 31399-31406.
  • Taraschi, T.F. and Beggs, J. (2000) A Head to Head Comparison of the Release of Amphotericin B from Abelcet and AmBisome in Human Plasma. J. Liposome Res. 10, 96-98.
  • Subang, R., Levine, J.S., Janoff, A.S., Davidson, S.M.K., Taraschi, T.F., Koike, T., Minchey, S.R., Whiteside, M., Tannenbaum, M. and Rauch, J. (2000) Phospholipid-bound beta-2-glycoprotein I induces the production of anti-phospholipid antibodies. J. Autoimmunity 15, 21-32.
  • Haltiwanger, B., Matsumoto, Y., Nicolas, E., Dianov, G.L., Bohr, V.A. and Taraschi, T.F. (2000) DNA base excision repair in human malaria parasites is predominantly by a long-patch pathway. Biochemistry 39, 763-72.
  • Trelka, D., Schneider, T., Reeder, J.C. and Taraschi, T.F. (2000) Evidence for vesicle-mediated trafficking of parasite proteins to the host cell cytosol and erythrocyte surface membrane in Plasmodium falciparum infected erythrocytes. Mol. Biochem. Parasitol. 106, 131-145.
  • Haltiwanger, B.M., Karpinich, N.O., and Taraschi, T.F. (2000) Characterization of class II AP endonuclease activities in the human malaria parasite, Plasmodium falciparum. Biochem. J. 345, 85-89.
  • Taraschi, T.F. (1999) Macromolecular transport in malaria-infected erythrocytes. In: Bock, G.R., Cardew, G. (eds) Transport and trafficking in the malaria-infected Erythrocyte. Novartis Found Symp. 226,114-120; discussion 121-125. Wiley, Chichester (Novartis Found Symp. 226)
  • Nicolas, E., Beggs, J., Haltiwanger, B., and Taraschi, T.F. (1998) A new class of DNA glycosylase/AP lyases that act on specific adenines in single-stranded DNA. J. Biol. Chem. 273, 17216-17220.
  • Taraschi, T.F., Trelka, D., Schneider, T.G. and Matthews, I. (1998) Plasmodium falciparum: Characterization of organelle migration during merozoite morphogenesis in asexual malaria infections. Exp. Parasitol. 88, 184-193.
  • Nicolas, E., Goodyer, I. and Taraschi, T.F. (1997) An Additional Mechanism of Ribosome-Inactivating Protein Cytotoxicity: Degradation of Extrachromosomal DNA. Biochem. J. 327, 413-417.
  • Nicolas, E., Beggs, J., Haltiwanger, B., and Taraschi, T.F. (1997). Direct Evidence for the Deoxyribonuclease Activity of the Plant Ribosome Inactivating Protein Gelonin. FEBS Lett. 406, 162-164.
  • Goodyer, I.D., Pouvelle, B., Schneider, T. G., Trelka, D. and Taraschi, T.F. (1997) Characterization of macromolecular transport pathways in malaria-infected erythrocytes. Mol. Biochem. Parasitol. 87, 13-28.
  • Baruch, D.I., Pasloske, B.L., Singh, H.B., Bi, X., Ma, X.C., Feldman, M., Taraschi, T.F. and Howard, R.J. (1995) Cloning the Plasmodium falciparum gene encoding PfEMP1, a malarial variant antigen and adherence receptor on the surface of parasitized human erythrocytes. Cell 82, 77-87.
  • Pouvelle, B., Gormley, J.A. and Taraschi, T.F. (1994) Characterization of trafficking pathways and membrane genesis in malaria-infected erythrocytes. Mol. Biochem. Parasitol. 66, 83-96.
  • Pouvelle, B., Farley, P.J., Long, C.A., and Taraschi, T.F. (1994) Taxol Arrests the Development of Blood-Stage Plasmodium falciparum in vitro and Plasmodium Chabaudi Adami in Malaria Infected Mice. J. Clin. Invest. 94, 413-417.
  • Gormley, J.A., Howard, R.J. and Taraschi, T.F. (1992) Trafficking of malarial proteins to the host cell cytoplasm and erythrocyte membrane involves multiple pathways. J. Cell Biol. 119, 1481-1495.
  • Pouvelle, B., Spiegel, R., Hsiao, L., Howard, R.J., Morris, R.L., Thomas, A.P. and Taraschi, T.F. (1991) Intraerythrocytic malaria parasites have direct access to serum macromolecules. Nature 353, 73-75.

Last Updated by KT Ferrera: Tuesday, May 14, 2013 10:34:25 AM

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