Faculty Affairs: Faculty Interests Database
Michael Holinstat, PhD

Medicine : Hematology
Associate Professor
Thomas Jefferson University
Department of Medicine
Division of Hematology
Cardeza Foundation for Hematologic Research

Associate Professor
Thomas Jefferson University
Department of Biochemistry and Molecular Biology
Mailing Address Contact Information
1020 Locust Street, 394 Jefferson Alumni Hall
Philadelphia, Pennsylvania 19107
United States
Phone: 215-955-6121
Fax: 215-955-9170
michael.holinstat@jefferson.edu
Qualifications
B.S., Southern Connecticut State University, Biology, 1995

M.S., University of Illinois at Chicago, Biological Sciences, 1999

Ph.D, University of Illinois at Chicago College of Medicine, Pharmacology, 2004

Postdoctoral Fellow, Vanderbilt University, Pharmacology, 2004-2008
Keywords
thrombosis; Rap1; GPCR; signal transduction; platelet; pharmacology; hematology; medicine; small G protein; signal transduction; signaling; lipoxygenase; 12-lipoxygenase; 12-HETE; 12-HETrE; fatty acid; oxidation; diabetes; T2DM; racial differences
Publications
  • Simon LM, Edelstein LC, Nagalla S, Woodley AB, Chen ES, Kong X, Ma L, Fortina P, Kunapuli S, Holinstat M, McKenzie SE, Dong JF, Shaw CA and Bray PF: Human platelet microRNA-mRNA networks associated with age and gender revealed by integrated plateletomics. Blood (paper in press), 2014.
  • Luci DK, Jameson II B, Yasgar A, Diaz G, Joshi N, Kantz A, Markham K, Perry S, Kuhn N, Yeung J, Kerns EG, Schultz L, Holinstat M, Nadler JL, Taylor-Fishwick DA, Jadhav A, Simeonov A, Holman TR and Maloney DJ. (2014) Synthesis and structure-activity relationship of 4-((2-hydroxy-3-methoxybenzyl)amino)benzenesulfonamide derivatives as potent and selective inhibitors of 12-lipoxygenase. J Med Chem; 57(2):495-506, 2014.
  • Tourdot BE, Ahmed I, Holinstat M: The emerging role of oxylipins in thrombosis and diabetes. Front Pharmacol. Jan; 4 (176):1-9, 2014.
  • Edelstein LC, Simon LM, Montoya RT, Holinstat M, Chen ES, Bergeron A, Kong X, Nagalla S, Mohandas N, Cohen DE, Dong JF, Shaw C and Bray PF: Racial differences in human platelet PAR4 reactivity reflects expression of PCTP and miR-376c. Nat Med; 19(12):1609-16, 2013.
  • Yeung J, Apopa PL, Vesci J, Stolla M, Rai G, Simeonov A, Jadhav A, Fernandez-Perez P, Maloney DJ, Boutaud O, Holman TR and Holinstat M: 12-lipoxygenase activity plays an important role in PAR4 and GPVI-mediated platelet reactivity. Thromb Haemost 110(3):569-81, 2013.
  • Edelstein LC, McKenzie SE, Shaw C, Holinstat MA, Kunapuli SP, and Bray PF: MicroRNAs in platelet production and activation. J Thromb Haemost. Jun; Suppl 1:340-50, 2013.
  • Ikei K, Yeung J, Apopa PL, Ceja J, Vesci J, Holman T.R.* and Holinstat M* (2012) Kinetic and cellular investigations of human platelet-type 12-lipoxygenases: Role of lipoxygenase products in platelet activation. J Lipid Res 53(12):2546-59.
  • Holinstat M, Hudson W, Colowick N.E., Blackemore D, Chen Q, Hamm H.E. and Cleator J.H (2012) Dichotomous effects of exposure to bivalirudin in patients undergoing percutaneous coronary intervention on protease-activated receptor-mediated platelet activation. J Thromb Thrombolysis; 35(2):209-222, 2012.
  • Yeung J and Holinstat M. (2012) Newer agents in antiplatelet therapy: a review. J Blood Med 3:33-42.
  • Yeung J, Apopa P.L., Vesci J, Kenyon V, Rai G, Patel M, Jadhav A, Simeonov A, Holman T, Maloney D, Boutaud O and Holinstat M. (2012) Protein kinase C regulation of 12-lipoxygenase-mediated human platelet activation. Mol Pharmacol 81(3):420-430.
  • Stefanini L, Boulaftali Y, Oullette T.D., Holinstat M, Desire L, Leblond B, Andre P, Conley P.B. and Bergmeier W.B. (2012) Rap1-Rac1 circuits potentiate platelet activation. Arterioscler Thromb Vasc Biol; 32(2):434-41.
  • Pawlinski R, Holinstat M (2011) We can do it together: PAR1/PAR2 heterodimer signaling in VSMCs. Arterioscler Thromb Vasc Biol. Dec; 31(12):2775-6.
  • Yeung J and Holinstat M. (2011) 12-lipoxygenase: a novel target for anti-platelet therapeutics. Cardiovasc Hematol Agents Med Chem. Jul; 9(3):154-64.
  • Kenyon V, Rai, G, Jadhav A, Schultz L, Armstrong M, Jameson JB II, Perry S, Joshi N, Bougie JM, Leister W, Taylor-Fishwick DA, Nadler JL, Holinstat M, Simeonov A, Maloney DJ and Holman TR: Discovery of potent and selective inhibitors of human platelet-type 12-lipoxygenase J Med Chem. Aug; 54(15):5485-97, 2011.
  • Holinstat M, Boutaud O, Apopa P.L, Vesci J, Bala M, Oates J.A. and Hamm H.E. (2011) Protease-activated receptor signaling in platelets activates cPLA2 differently for cyclooxygenase-1 and 12-lipoxygenase catalysis. Arterioscler Thromb Vasc Biol. Feb; 31(2):435-442.
  • Minshall RD, Vandenbroucke EE, Holinstat M, Place AT, Tiruppathi C, Vogel SM, Van Nieuwamerongen GP, Mehta D, Malik AB. (2010) Role of protein kinase Czeta in thrombin-induced RhoA activation and inter-endothelial gap formation of human dermal microvessel endothelial cell monolayers. Microvasc Res. Sep; 80(2):240-249.
  • Holinstat M, Preininger A, Milne S.B., Hudson, W.J., Brown H.A. and Hamm H.E. (2009) Irreversible platelet activation requires PAR1-mediated signaling to phosphatidylinositol phosphates. Mol Pharmacol. Aug; 76(2): 301-313.
  • Voss B, McLaughlin J, Holinstat M, Roy Zent and Hamm H.E. (2007). PAR-1, but not PAR-4, activates human platelets through a Gi/o/PI3K signaling axis. Mol Pharmacol. Feb; 71(5): 1399-1406.
  • Holinstat M, Voss B, Bilodeau M.L and Hamm H.E. (2007) Protease activated receptors regulate human platelet activation through a phosphatidic acid-dependent pathway. Mol Pharmacol. March; 71(3): 686-694.
  • Holinstat M, Oldham W and Hamm H.E. (2006) G protein coupled receptors: Evolving views on physiological signaling. EMBO J. Sep; 7(9):866-9.
  • Holinstat M, Voss B, Bilodeau M.L, McLaughlin J, Cleator J and Hamm H.E. (2006) PAR4, but not PAR1, signals human platelet aggregation via Ca2+ mobilization and synergistic P2Y12 receptor activation. J Biol Chem. Sep 8; 281(36):26665-74.
  • Holinstat M, Knezevic N, Broman M, Samarel A.M, Malik A.B and Mehta D. (2006) Suppression of RhoA activity by focal adhesion kinase-induced activation of p190RhoGAP: Role in regulation of endothelial permeability. J Biol Chem. Jan 27; 281(4):2296-2305.
  • McLaughlin J.N, Shen L, Holinstat M, Earles L and Hamm HE. (2005) Functional selectivity of G protein signaling by agonist peptides and thrombin for the protease-activated receptor-1. J Biol Chem. Jul 1;280(26):25048-59.
  • Park T.J, Klug A, Holinstat M, Grothe B. (2004) Interaural Intensity Difference Processing in the Lateral Superior Olive and Inferior Colliculus. J. Neurophys. July 92 (1):289-301.
  • Ahmmed GU, Mehta D, Vogel S, Holinstat M, Paria B, Tiruppathi C and Malik A.B. (2004) Protein Kinase C-± phosphorylates transient receptor potential channel-1 (TRPC1) and regulates store operated Ca2+ entry. Role in signaling increased endothelial permeability. J. Biol. Chem. April 279 (20):20941-49.
  • Mehta D, Ahmmed GU, Paria B, Holinstat M, Voyno-Yasenetskaya T, Tiruppathi C, Minshall RD and Malik AB. (2003) RhoA interaction with inosotol 1,4,5-triphosphate receptor and transient receptor potential channel-1 regulates Ca2+entry. Role in signaling increased endothelial permeability. J. Biol. Chem. Aug 29;278 (35):33492-500.
  • Holinstat M, Mehta D, Kozasa T, Minshall R.D, Malik A.B. (2003) Role of PKC±-induced phosphorylation in activation of p115RhoGEF and endothelial cytoskeletal rearrangement. J. Biol. Chem. Aug 1; 278 (31):28793-28798.
  • Mehta D, Tiruppathi C, Sandoval R, Minshall RD, Holinstat M, Malik AB. (2002) Modulatory role of focal adhesion kinase in regulating human pulmonary arterial endothelial barrier function. J. Phys. 539 (Pt 3):779-789.

Last Updated by Michael Holinstat, PhD: Thursday, December 20, 2012 2:54:07 PM

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